Dr. Igor M. Sauer: obtained postdoctoral lecture qualification (Habilitation) in Surgery


Doors of the Roadrunner's Paradise will open at 9 p.m. - Emigrantski Raggamuffin by Rotfront ahead!

Management of Acute Kidney Problems

PhD Student Award in Regenerative Medicine

Acute kidney failure is an important clinical area in the intensive care unit setting. An estimated 5–20% of critically ill patients experience an episode of acute kidney failure during the course of their illness, and about 5% of patients admitted to an ICU will eventually require renal replacement therapy. In these patients, in-hospital mortality is extremely high, exceeding 50%. Thus, the early detection and causal treatment of acute kidney problems is vitally important for a successful outcome. Written by internationally renowned experts, this clinical reference offers helpful advice with the most recent information on definition, epidemiology, pathophysiology, clinical causes of acute kidney failure, differential diagnostic approaches for patients with acute renal failure, and various key aspects related to the adequate delivery of acute renal replacement therapy.
It also gives a detailed outline of important measures for their clinical management. This reference is intended as a helpful guide for all clinicians involved in the care of patients at risk of developing acute kidney problems.

The book is written for all clinicians who are involved in the care of patients at risk of developing acute kidney problems; e.g. fellows and residents in nephrology, intensive care, internal medicine, anaesthesiology, surgery, paediatrics, diagnostic and interventional radiology, urology, cardiology and clinical immunology.

Associated Investigator of BCRT

The Berlin-Brandenburg School for Regenerative Therapies (BSRT) offers young talented scientists in Berlin and Brandenburg:
Best Publication Award: The award will be granted for the most outstanding paper for progress in Regenerative Medicine accepted by a peer review journal in the last two years. The award comprises 1.500 Euro.
Best Presentation Award: The award will be granted for the best poster presented at an international conference with a peer review system within the last two years. The award comprises 500 Euro.
Info & Contact: BSRT, Charité Campus Virchow-Klinikum, Augustenburger Platz 1, 13353 Berlin, E-Mail: award@bsrt.de, Web: www.bsrt.de

One Day on the Liver 2008 - Programme

The Steering Committee of the Berlin-Brandenburg Center for Regenerative Therapies (BCRT) decided to involve Igor Sauer and his group as an Associated Investigator (AI).
The BCRT is a cooperative research institution of the Charite University Hospital in Berlin and Germany's largest research association, the Helmholtz Association. BCRT also receives generous financial support from the BMBF and the states of Berlin and Brandenburg, as well as from the Technology Foundations in Berlin and Brandenburg, the Future Fund Berlin and from various industry partners. More than 15 regional partners from science and industry are active members of the consortium at the BCRT.

ESAO One Day on the Liver 2008 in Geneva


Dr. med. Florian W.R. Vondran

As all the recent years the “One day on the liver” – a day dedicated to liver support systems and therapeutic strategies in the case of a failing liver - will take place during the ESAO 2008 meeting in Geneva. For the first time we organised the "Young investigators' Liver Session". We asked the heads of several groups working in the field to send one of their best students to present their latest results. This session is dedicated to PhD students and is chaired by PhD students involved in liver support research. Of course all participants of the meeting are invited to attend!
The ESAO One Day on the Liver 2008 (ODOL) will take place Thursday, Sept. 4th, 2008.

Imaging of human hepatocytes via MPIO and MRI

Florian Vondran successfully defended his thesis "summa cum laude".
After years of extremely fruitful research in the field of liver support, hepatocyte isolation and cryopreservation he is (co-)author of 7 papers in peer reviewed journals.

Tat-peptide modified MPIO

Nathanel Raschzok's and Haluk Morgül's manuscript entitled "Imaging of Primary Human Hepatocytes Using Micron-Sized Iron Oxide Particles and Clinical Magnetic Resonance Tomography" has been accepted for publication in the Journal of Cellular and Molecular Medicine (impact factor: 6,55). Authors are Nathanael Raschzok, Mehmet H. Morgul, Jens Pinkernelle, Florian W.R. Vondran, Nils Billecke, Nora N. Kammer, Gesine Pless, Michaela K. Adonopoulou, Christian Leist, Lars Stelter, Ulf Teichgraber, Ruth Schwartlander and Igor M. Sauer. Nathanael Raschzok and Mehmet Haluk Morgul contributed equally to this work. The contribution of Ruth Schwartländer has to be emphasised as well. Transplantation of primary human hepatocytes is a promising approach in certain liver diseases. For visualisation of hepatocytes during and following cell application and the ability of a timely response to potential complications, a non-invasive modality for imaging of the transplanted cells has to be established. The aim of this study was to label primary human hepatocytes with micron-sized iron oxide particles (MPIOs), enabling the detection of cells by clinical magnetic resonance imaging (MRI). Primary human hepatocytes isolated from 13 different donors were used for labelling experiments. Following dose finding studies, hepatocytes were incubated with 30 particles/cell for 4 hours in adhesion culture. Particle incorporation was investigated via light, fluorescence and electron microscopy and labelled cells were fixed and analysed in an agarose suspension by a 3.0 Tesla MR scanner. Hepatocytes were enzymatically resuspended and analysed during a five-day reculture period for viability, total protein, enzyme leakage (AST, LDH) and metabolic activity (urea, albumin). A mean uptake of 18 particles/cell could be observed, and primary human hepatocytes were clearly detectable by MR instrumentation. The particle load was not affected by resuspension and showed no alternations during the culture period. Compared to control groups, labelling and resuspension had no adverse effects on viability, enzyme leakage and metabolic activity of human hepatocytes. Conclusion: The feasibility of preparing MPIO-labelled primary human hepatocytes detectable by clinical MR equipment was shown in vitro. MPIO-labelled cells could serve for basic research and quality control in the clinical setting of human hepatocyte transplantation.

Isolation of primary human hepatocytes

Haluk Morgül and Nathanael Raschzok published their first results on "Tracking of primary human hepatocytes with clinical MRI: Initial results with Tat-peptide modified superparamagnetic iron oxide particles." in the March issue of IJAO (Int J Artif Organs 2008, 31:252-257): The transplantation of primary human hepatocytes is a promising approach in the treatment of specific liver diseases. However, little is known about the fate of the cells following application. Magnetic resonance imaging (MRI) could enable real-time tracking and long-term detection of transplanted hepatocytes. The use of superparamagnetic iron oxide particles as cellular contrast agents should allow for the non-invasive detection of labelled cells on high-resolution magnetic resonance images. Experiments were performed on primary human hepatocytes to transfer the method of detecting labelled cells via clinical MRI into human hepatocyte transplantation. For labelling, Tat-peptide modified nano-sized superparamagnetic MagForce particles were used. Cells were investigated via a clinical MR scanner at 3.0 Tesla and the particle uptake within single hepatocytes was estimated using microscopic examinations. The labelled primary human hepatocytes were clearly detectable by MRI, proving the feasibility of this new concept. Therefore, this method is a useful tool to investigate the effects of human hepatocyte transplantation and to improve safety aspects of this method.