Future Medicine 2017

FOM17_Logo_RZ_TZ_Unterzeile

What’s trending? What’s new in health science? To find out, please save the date for the second Future Medicine in Berlin on November 7, 2017.

Tagesspiegel and Berlin Institute of Health, together with Charité - Universitätsmedizin Berlin and Max Delbrück Center for Molecular Medicine, will feature outstanding international scientists, great visions of the future of medicine, and an exceptional concentration of knowledge.
The four sessions of Future Medicine 2017 will be:
  • Digital Health and Big Data
  • Precision Medicine and Predictive Models
  • Cell and Gene Therapies
  • Stem Cells and Human Disease Modeling

Simon Moosburner, a student of medicine interested in regenerative medicine and future technologies, will talk about “Virtual & Mixed Reality: The Next Milestone in Surgery?” at Future Medicine 2017.

21. Chirurgischen Forschungstage

CFT-Header_lang_05_low

The 21. Chirurgischen Forschungstage took place in Cologne. Five of our students gave terrific presentations:
S. Moosburner gave an oral presentation on „Steatotic Liver Transplantation – a Growing Problem with Severe Complications“, H. Everwien on „Different biological scffolds as a platform for engineering an endocrine Neo-Pancreas by using decellularization and recellularization techniques“, M. Noesser on „A comprehensive description of the development of a stable closed circuit for ex vivo rat liver machine perfusion“, R. Horner on „Is Percoll purifcation necessary for isolation of primary human hepatocytes?“, and N. Seiffert on „Recellularization of Decellularized Bovine Carotid Arteries using Human Endothelial Progenitor Cells: One Step towards an Autologous Bypass Graft“.

ESOT 2017: The future of transplantation

esot2017
The ESOT YPT Workshop and its Presidential Debate gives you a chance to engage with a variety of speakers in a lively ‘town hall’ format, as well as connecting with other young transplant professionals from around the world. The session will begin with talks from three key presenters on the past, present, and future of transplantation and open Q&As with each presenter.



09:00– 10:35 - The future of transplantation: 3 lectures on Past, Present and Future

CHAIRS: Alice Koenig, Lyon, France
Thomas Resch, Innsbruck, Austria

09:05 Lecture: The future of transplantation - Historical Perspective
Sir Roy Calne, Cambridge, United Kingdom

09:25 Open Q&A with Roy Calne

09:35 Lecture: The future of transplantation - Present perspective
Jan Lerut, Brussels, Belgium

09:55 Open Q&A with Jan Lerut

10:05 Lecture: The future of transplantation - Future perspective
Igor Sauer, Berlin, Germany

10:25 Open Q&A with Igor Sauer

International Symposium organized by SFB738

International_Symposium_Flyer_20170623

Charité BIH Entrepreneurship Summit

image001
The Charité BIH Entrepreneurship Summit is a preeminent international cross-disciplinary forum for sharing and exploring the most important discoveries and emerging trends influencing the future of healthcare around the world.

Every year in May over 400 global leaders in healthcare innovation, including entrepreneurs, scientists, physicians, investors, policymakers, and business leaders convene at the Charité BIH Entrepreneurship Summit in the vibrant German capital of Berlin. We offer our participants a one-of-a-kind opportunity to meet with world-class specialists working at Charité & MDC, to build relationships with prominent international partners and experts working in the healthcare industry, and to help grow businesses.

This year's 10th Charité Entrepreneurship Summit will again take place at the Berlin-Brandenburg Academy of Sciences and Humanities on May 8 - 9, 2017. The Summit is significantly supported by the Berlin Institute of Health and focusses on 'Global Challenges of Healthcare'. Israel will be the official partner country for the Summit 2017. We are looking forward to learning more about the Israeli innovation & start-up culture, funding opportunities and challenges in Healthcare.

The Summit's two-day agenda will address a wide variety of topical issues including change of innovation culture, healthy aging & degenerative diseases, virtual reality and mental health. In addition, the Summit will seek to engage participants in lively discussions about business, science and the intersection of the two. Startups and Entrepreneurs are invited to apply for the LifeSciences VentureMarket, a platform to present their companies to a pool of international angels, venture investors, and corporate funds at this year's Summit.

You will find the program here.

imag|Ne surgery – April 28-29, 2017

imagINe surgery_third announcementimagINe surgery_third announcement

Einstein Center Digital Future – Opening



C8UOe6fWsAAkLPc

Poster_Programm

Forschungs-Colloquium I 2017

FC2017

Programm–2017

BIH Charité Clinician Scientist Symposium

CSS_Flyer_196423882

Decellularization of pancreata – EPITA Award

IMG_5485
Ben Strücker presented our latest results on DECELLULARIZATION OF WHOLE RAT PANCREATA – EVALUATION OF THREE DIFFERENT PERFUSION ROUTES at the 5th EPITA Winter Symposium in Innsbruck from the 25th to the 27th of January 2015. He received the AIDPIT&EPITA Award for the best oral presentation.
Congratulations!

Ben Strücker presented three effective protocols for rat pancreas perfusion decellularization, evaluating different perfusion routes. In contrast to liver decellularization the perfusion route seems to have no major impact on decellularization results. The dPECMs could serve for cellular repopulation with islets from a different (xenogene) origin to generate functional, transplantable endocrine pancreata in vitro.

Forschungs-Colloquium I – 2015

Das erste gemeinsame Forschungs-Colloquium der Chirurgischen Klinik an den Standorten CCM und CVK im Jahr 2015 findet im Hörsaal der Pathologie im Erdgeschoss des Forschungshauses, Charité – Campus Virchow Klinikum, Forum 4, am 10 Januar 2015 von 10:00 Uhr bis 17:30 Uhr statt. Forschungs-Colloquium

ESAO 2015 in Leuven, Belgium

image

Presentations at DTG 2014

111111
Nathanael Raschzok presented his poster „Protein biomarkers for diagnosis and prediction of acute cellular rejection after liver transplantation“, Benjamin Strücker his work entitled „Oszillierende Druckschwankung verbessern signifikant die Ratten- und Schweineleber Dezellularisierung“ and Rosa Schmuck her posters „Risk of postoperative infections after LTX rises with MELD score“ and „Protein biomarkers in bile as a diagnostic tool after liver transplantation“.

5th EPITA Winter Symposium

epita_880x240

25th European Students‘ Conference

esc The ESC is one of the largest student-run biomedical conferences worldwide. It was established in 1989 as one of the first student conferences facilitating exchange between Western and Eastern Europe. Until today, it aims to foster international understanding within the young scientific community, and to promote high-quality science among young researchers in the biomedical field.

This year’s lecture series “Rethinking Medical Research – how do we achieve innovation?” will explore the dichotomy of medical advances by looking at both the opportunities and challenges that are presented to researchers and physicians. With topics ranging from open access policies, pharmaceutical innovations, and revolutionary health policies to advances in global health – we hope that this year’s topic will both challenge and inspire our participants!
The conference will take place from September 17th – 20th at the Virchow Klinikum Campus of the Charité Universitätsmedizin in Berlin, Germany.

More information here!

WTC 2014 in San Francisco

WTC2014

Ben Strücker presented the latest results on „Perfusion Decellularization of Pig and Rat Livers Applying Undulating Surrounding Pressure Conditions“. Co-authors were A. Butter, K. Hillebrandt, R. Voitl, D. Polenz, A. Reutzel-Selke, K. Joehrens, D. Geisel, N. Raschzok, P. Neuhaus, J. Pratschke and I.M. Sauer.

Nathanael Raschzok presentation was entitled: „Perioperative Serum Levels of CXCL9 and CD44 Predict the Risk of Acute Cellular Rejection in Liver Graft Recipients“. Co-authors were N. Raschzok, A. Reutzel-Selke, R. Schmuck, M. Morgul, M. Bartels, G. Puhl, D. Seehofer, M. Bahra, A. Pascher, P. Neuhaus, J. Pratschke and I.M. Sauer.

Charité Clinical Scientist Summer Symposium

CCS_2014

Please check the program here...

yESAO 2014

yesao2014_program_rome

„Biologisierte Medizintechnik“

callforpaper_cover
Die Berlin Partner für Wirtschaft und Technologie, Bereich Medizintechnik, die Charité - Universitätsmedizin Berlin und die IHK Berlin laden zum
 28. Treffpunkt Medizintechnik „Biologisierte Medizintechnik“
am Donnerstag, 25. September 2014, 9 bis 17 Uhr
in der Charité – Universitätsmedizin Berlin, Campus Virchow-Klinikum
ein.
Mit dem „Treffpunkt Medizintechnik“ wird eine interdisziplinäre Dialogplattform für Klinik, Wissenschaft und Wirtschaft geboten.
Die Deadline für Vortragsmeldungen ist der 11. April 2014. Unter www.healthcapital.de/TP-Med finden Sie weitere Informationen und haben die Möglichkeit, sich auch online anzumelden.

41st Annual Congress of the ESAO in Rome

head_logo
Prof. Celestino Pio Lombardi, Gemelli Polyclinics, Catholic University of the Sacred Heart in Rome, and Prof. Gerardo Catapano organize the 41st Annual Congress of the European Society for Artificial Organs (ESAO) in Rome, Italy.

The 41st ESAO Congress will be focused on “Patient happiness: the Holy Grail of organ substitution”, and will be held on September 17-20, 2014 at the Giovanni XXIII congress center located inside the Gemelli Polyclinics in Rome, Italy. The meeting is expected to attract aprox. 600 participants with both clinical and technical expertise from all over the world.
The objective of the Congress is to bring together scientists with different backgrounds working at the development, optimization and translation to the clinics of treatments of organ deficits based on the use of artificial, bioartificial, and cell-based organs or prostheses, and to discuss the importance of technical, psychological and ethical issues to the happiness of patients with serious tissue or organ deficits so as to re-define the design criteria of devices and treatments.
Please, check the ESAO website (www.esao.org) for the actual availability of the Congress website (www.esao2014.org).

Transforming Transplantation – Expert Summit 2013

‘Transforming Transplantation: The Expert Summit 2013’ is taking place in Madrid on the 21-22 November.
 
The main objective of the summit is to provide a cutting-edge educational opportunity to discuss and learn about new developments in the field from transplantation experts.TT2013 AGENDA

Retreat 2013

ESAO 2013 in Glasgow

IMG_1194
The XXXX ESAO Congress took place in Glasgow, September 11th - 14th, 2013. This year we had three oral presentations focussing on our latest results in decellularization and recellularization of the liver. Ben Strücker presented our latest results on „Improved rat liver decellularization by arterial perfusion under oscillating surrounding pressure“. Antje Butter and Karl Hillebrandt gave two oral presentations during the yESAO Rapid Fire Session on „Proprietary rat liver decellularization device utilizing oscillating surrounding pressure to improve micro perfusion within the organ“ and Pig liver decellularisation through portal vein and hepatic artery perfusion under oscillating surrounding pressure“.
Furthermore, Karl Hillebrandt received the „2. Prize for the best Poster“!

ESOT 2013 in Vienna

ESOT2013
Martina Mogl gave an oral presentation on „Characterization of cells isolated from explanted diseased human livers and evolution after cold storage“ at this year’s 16th Congress of the European Society for Organ Transplantation (ESOT) in Vienna.

12th Congress of the Cell Transplant Society

cts2013_header

The 12th Congress of the Cell Transplant Society tookplace in Milan, Italy, from July 7 to 11, 2013.
Nathanael Raschzok gave a presentation on "Loco-regional detection and stimulation of transplanted liver cells by particle-based miRNA depletion" and Martina Mogl on "isolation of adult hepatocytes and progenitor cells from explanted diseased human livers“.

IEEE Engineering in Medicine and Biology Society

header
The 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC’13) will take place 3-7 July 2013, at the Osaka International Convention Center, in Osaka, Japan.
The conference will cover diverse topics such as biomedical engineering, healthcare technologies, and medical and clinical applications.
The ESAO will be represented via a minisymposium entitled "Artificial Organs for Metabolic Support. The most Challenging Problems". Jan Wojcicki will give a presentation on "Artificial Organs for Metabolic Support: The Most Challenging Problems of Artificial Pancreas", Bernd Stegmayr on "Artificial Organs for Metabolic Support: The Most Challenging Problems in Severe Kidney Injury When Dialysis Is Necessary" and Igor Sauer on "Artificial Organs for Metabolic Support: The Most Challenging Problems of Liver Support".

Einladung zur öffentlichen Abschlußpräsentation

EFRE
Sehr geehrte Damen und Herren,
Dear ladies and gentlemen,

Sie sind herzlich eingeladen, unserer öffentlichen Abschlusspräsentation unseres EU/EFRE-Projekts am 20.03.2013 um 15:00 Uhr beizuwohnen. Zusammen mit unseren Partnern der Firma microparticles GmbH werden wir den aktuellen Stand zur „Entwicklung von Partikel zur Detektion und ultralokoregionären Stimulation transplantierter Leberzellen“ darlegen.
You are cordially invited to attend our public presentation of our EU/EFRE project. Together with our partners of microparticles GmbH we will present our latest results concerning the „Development of particles for detection and ultralocoregional stimulation of transplanted liver cells“.

Wann/When?
20.03.2013 um/at 15:00

Wo/Where?
Experimentelle Chirurgie und Regenerative Medizin
Klinik für Allgemein-, Viszeral- und Transplantationschirurgie
Charité, Campus Virchow-Klinikum
Forschungshaus/BMFZ
Pilzraum, 1.OG

Wir wären dankbar, wenn Sie Ihr Kommen via email (anja.selke@charite.de)bestätigen könnten.
RSVP via email (anja.selke@charite.de).

GASL 2013 Poster Prize

logo
Nathanael Raschzok's poster entitled "Serum protein biomarkers for acute rejection
in liver transplantation" was awarded with the GASL 2013 poster prize during the 29th Annual Meeting of the German Association for the Study of the Liver (GASL) in Hannover, Germany. Co-authors are Kukuh A. Prabowo, Anja Reutzel-Selke, Rosa B. Schmuck, Mehmet H. Morgul, Laura M. Tannus, Stephanie König, Sven Jonas, Peter Neuhaus, and Igor M. Sauer.


Although 20–40% of patients experience at least one episode of acute rejection (AR) after liver transplantation (LTx), diagnosis of AR is still mainly based on tissue analysis from liver biopsies. Biomarker routinely taken from blood samples would be a powerful non-invasive tool for monitoring of the liver graft status. The aim of this study was to investigate the diagnostic and prognostic value of serum protein biomarkers for acute rejection in LTx recipients. Serum samples from n=20 patients with AR and n=15 stable controls were analyzed. CXCL9 and CD31 were up-regulated in AR samples compared to controls at the time point of histologically proven rejection and at earlier time points prior rejection. Areas under the Receiver Operation Characteristics (ROC) curves were 0.6 and 0.7 at the day of rejection and 0.8 at POD1 for CXCL9 and CD31, respectively. IL-6 was increased prior and during rejection, while CD44 showed an opposite trend.
Serum protein biomarkers could be valuable for detection and prediction of AR after LTx. However, a larger number of patients, additional control groups, and prospective clinical trials will be necessary to proof the clinical utility of this diagnostic tool.

Moreover, Rosa Schmuck presented her data on "Bile: miRNA pattern and cell morphology as a diagnostic tool after liver transplantation" (co-authors: Nathanael Raschzok, Anja Reutzel-Selke, Steffen Lippert, Stephanie König, Kukuh A. Prabowo, Mehmet H. Morgul, Laura M. Tannus, Sven Jonas, Peter Neuhaus, and Igor M. Sauer) and Luisa Lisboa her work on "MicroRNA miR-352 in early liver regeneration: what role?" (co-authors: Nathanael Raschzok, Annekatrin Leder, Natalie Schlüter, Marc Jörres, Susanne Kolano, Antje Butter, Steffen Lippert, Wiebke Werner, Peter Neuhaus, and Igor M. Sauer).

XXXX ESAO Congress in Glasgow

esao2013
The European Society for Artificial Organs (ESAO) invites you to the XXXX ESAO Congress of the society to be held in Glasgow (Scotland, UK), September 11th-14th 2013.The motto of the ESAO congress will be 'lab to patient - from concept to treatment.



Latest information is available via www.esao2013.org !

Retreat 2012

Presentations at ESAO 2012 in Rostock, Germany

CN2K8211
This year members of the team gave the following presentations:

“Liver: Current regenerative strategies and future solutions for the liver" (N. Raschzok, oral presentation)

"Neohybrid liver graft - a novel concept of in vivo tissue-engineering" (S. Rohn, oral presentation)

"Micro RNAs in liver regeneration: the mysterious MIR-352" (L. Lisboa, oral presentation)

"Micron-sized iron oxide particles for detection and loco-regional stimulation of transplanted liver cells" (A. Leder, oral presentation)

"Prospective validation of cross organ serum protein biomarkers – Initial results with CXCL9 and CD44 for diagnosis of acute liver rejection" (K.A. Prabowo, poster presentation)

TTS 2012 – Thank you!

Theater02
The Science Circuses are located at each end of the Exhibition Area. You will find the Mini-Theatres for the Mini-Oral Sessions here as well as the Poster Lounges and the Web Stations. Science Circus I includes Mini-Theatres 1 – 5; Science Circus II includes Mini-Theatres 6 – 10. In each Science Circus you will find Web Stations where you can access the web as well as the ePosters.
Via wireless headphones and dedicated channels for each mini-theater this is – to our knowledge – the first successful concept for mini-oral presentations!
Thanks to m-events and Interplan for the tremendous support!

TTS 2012 | Over 500 presentations online !

The LOC would like to thank all participants for a tremendous 24th International Congress of The Transplantation Society in Berlin!
2268 abstracts were submitted and more than 4800 delegates from 94 countries attended the congress!
The Postgraduate Weekend offered 14 workshops with 45 speakers. Beside 29 Sunrise Symposia with 103 Speakers, 34 State of the Art Sessions with 138 speakers and five Plenary Sessions (16 speakers) we had 53 sessions with 465 oral presentations and 37 sessions with 287 mini oral presentations !
Furthermore, we would like to thank Astrid Enke, Lena Dochat and Stefanie Rensch (Interplan) and the technical experts at m-Events for their excellent work !
As Science and Medicine is nothing without vivid life we would like to thank Rotfront, Berlin Comedian Harmonists and the Capital Dance Orchestra for their memorable performances!


2012-07-1509772012-07-1510022012-07-1510442012-07-1611052012-07-1611542012-07-1703242012-07-1833282012-07-1833972012-07-1834082012-07-1834122012-07-1834202012-07-1834402012-07-1834602012-07-183504

All the great photos by Jan Pauls.
The organ sculptures were made by Jan Pareike.

ESAO 2012 – Final Program Online !

2012-07-160085
The Transplantation Society is developing a comprehensive educational program for residents, fellows, transplant specialists and related professionals.

The program will now include more than 500 presentations by international experts in all aspects of basic biology and clinical transplantation give during the 24th International Congress of the Transplantation Society
You may access the videos here !

TTS 2012 | Presentations

Based on his scientific abstract on “Silica-Based Micron-Sized Iron Oxide Particles for Detection and Loco-Regional Stimulation of Transplanted Liver Cells”, Nathanael Raschzok (and Igor M. Sauer) received the TTS­-DTG International Basic Science Mentee/Mentor Award for the 24th International Congress of The Transplantation Society, in Berlin, Germany. The Basic Science Mentee/Mentor award recognizes the efforts of basic scientists, who have advanced our understanding of transplant science/immunobiology and/or treatment of transplant recipients, as well as the importance of young investigators in furthering the basic science of transplantation.

Congratulations !

TTS 2012 | Science Circus

Martina Mogl presented "Neohybrid Liver Graft - a Novel Concept of in Vivo Tissue-Engineering"

Rosa Schmuck presented our first results concerning "miRNA Pattern Within the Bile as a Diagnostic Tool after Liver Transplantation"

Nathanael Raschzok gave an oral presentation entitled "Silica-Based Micron-Sized Iron Oxide Particles for Detection and Loco-Regional Stimulation of Transplanted Liver Cells"

Haluk Morgül gave a talk on "MicroRNA as Biomarker for Diagnosis and Prediction of Recurrence of Human Hepatocellular Carcinoma after Liver Transplantation - Preliminary Results from a Multicenter Database"

Furthermore, the group presented a poster concerning "Prospective Validation of Serum Protein Biomarkers for Detection of Acute Liver Rejection – Initial Results with CXCL9 and CD44"

TTS 2012 | Postgraduate Weekend

app-preview
badge_appstore-lrg
Click here to download the TTS App for your iPhone or iPad

android_app_on_play_logo_small
Click here to download the TTS App for your Android Phone

The free TTS Congress App gives users the opportunity to:
• Browse, search and view the Scientific Programme by Speakers, Session, Topics
and Keywords
• Access the Floor Plan to easily find Exhibitors and other meeting locations onsite
• Create your personal schedule

TTS 2012 | Congress App available !


TTS_HauptProrgamm_2012_high 00001


TTS 2012: Click here to download the On-Site Program as PDF File pdf

TTS 2012 | On-Site Program available !

feier
Today, Nora N. Kammer and Kristen Steinz successfully defended their thesis!

Nora defended her thesis "summa cum laude". She presented her results on labelling of primary human hepatocytes with micron-sized iron oxide particles in suspension culture suitable for large-scale preparation. In German her thesis is entitled „Markierung primärer humaner Hepatozyten mit mikroskaligen Eisenoxidpartikeln in temporärer Suspensionskultur“.
Kirsten defended her thesis „magna cum laude“. Her work is entitled „Evaluation der Applikationsrouten für die Leberzelltransplantation im Großtiermodell“


Congratulations!

XXXIX. ESAO Congress in Rostock, Germany

TTS2012_AbstractMeeting

More than 2000 abstract were submitted for the TTS 2012 congress in Berlin. Members of all program committees will meet in Berlin for two days in order to define the Oral, Mini-Oral and Poster sessions.

TTS 2012 - Abstract Meeting in Berlin

2008_1

TTS 2012: Abstract submission now open !

hhtts12_header

Please join the mailing list and follow us via Twitter @TTS2012 for latest information concerning the 24th International Congress of The Transplantation Society to be held in Berlin, Germany from July 15th - 19th 2012 !

TTS 2012: Follow us via Twitter !

stacks_image_35_1
The 24th International Congress of The Transplantation Society to be held in Berlin, Germany from July 15th - 19th 2012 is designed for physicians, surgeons, scientists and organ procurement personnel, who are interested in clinical and research aspects of solid organ, cell and tissue transplantation. The program is developed to encourage the exchange of new scientific and clinical information, and support an interchange of opinions regarding care and management issues, as well as socioeconomic, ethical and regulatory issues relevant to transplantation.
In addition to the classical types of scientific sessions including plenary sessions, symposia, workshops and poster presentations, we will also offer new types of scientific sessions within the Forum Futurum.
The Forum Futurum is a forum integrated in the congress that will take place on three days. Each day is assigned to a topic and the forum is dedicated to cutting-edge science in our field. The topics are Regenerative Medicine, Imaging and Tailored Pharmacotherapy.
The Forum Futurum is designed to be a highly interactive communication space where companies can introduce their products and conversations about the latest research are stirred. In a futuristic environment the Forum Futurum contributes to an extraordinary TTS 2012 Exhibition experience and will provide exhibitors the opportunity to highlight new product information to physicians and health care professionals. Enhance the educational element of your company's noteworthy products and services with a presentation in the Forum Futurum. The Forum Futurum provides exhibitors with a unique and effective marketing opportunity to hold live promotional presentations or activities designed to raise awareness of featured services and products.
The Forum Futurum will make its debut in the TTS 2012 Exhibit Hall, featuring experts from companies and scientific groups providing state-of-the-art content-led information about new technologies, products and ideas. The Forum Futurum will be open July 16th – July 18th. Every day is dedicated to one emerging field in transplantation:
Monday, July 16th, 2012: One Day on
Regenerative Medicine
Tuesday, July 17th, 2012: One Day on
Imaging
Wednesday, July 18th, 2012: One Day on
Tailored Pharmacotherapy

More information via
www.forum-futurum.org and www.transplantation2012.org !

TTS 2012: www.transplantation2012.org is online!

bme_summit_sep_2007Information via www.stiftung-charite.de/entrepreneurship .

Presentations at this year's XXXVII ESAO Congress

6a00d83452732869e20134886f0b7f970c-120wi
Hannelore Ringe published the investigations concerning the applicability, efficacy, and safety of single-pass albumin dialysis in children ("Continuous veno-venous single-pass albumin hemodiafiltration in children with acute liver failure." Pediatr Crit Care Med. 2010 Oct 1. [Epub ahead of print]). Co-authors are Varnholt V, Zimmering M, Luck W, Gratopp A, König K, Reich S, Sauer IM, Gaedicke G, and Querfeld U.
The paper reports on a retrospective data review of uncontrolled clinical data of an university-based pediatric intensive care unit collaborating with a local center for liver transplantation.  Nine children, aged 2 to 15 yrs, who were treated with single-pass albumin dialysis for acute liver failure of various origins under a compassionate-use protocol between 2000 and 2006. All patients met high-urgency liver transplantation criteria. Single-pass albumin dialysis was performed as rescue therapy for children with acute liver failure. The decrease in hepatic encephalopathy (grades 1-4) and the serum levels of bilirubin, bile acids, and ammonium were measured to assess the efficacy of detoxification. As a measure of liver synthesis function, thromboplastin time and fibrinogen were analyzed. The safety of the procedure was assessed by documenting adverse effects on mean arterial blood pressure, platelet count, and clinical course. Seven out of nine patients were bridged successfully to either native organ recovery (n = 1) or liver transplantation (n = 7), one of them twice. Six out of nine patients undergoing single-pass albumin dialysis (ten treatments) survived. In six patients, hepatic encephalopathy could be reduced at least by one degree. Ammonium, bilirubin, and bile acid levels decreased in all patients. One patient had an allergic reaction to albumin. In childhood acute liver failure, treatment with single-pass albumin dialysis was generally well tolerated and seems to be effective in detoxification and in improving blood pressure, thus stabilizing the critical condition of children before liver transplantation and facilitating bridging to liver transplantation. It may be beneficial in avoiding severe neurologic sequelae after acute liver failure and thereby improve survival. Single-pass albumin dialysis is an inexpensive albumin-based detoxification system that is easy to set up and requires little training. Whether and to what extent single-pass albumin dialysis can support children with acute liver failure until native liver recovery remain unclear.

XXIII International Congress of TTS

6a00d83452732869e20134886f1cfc970c-800wi
At this year's XXXVII ESAO Congress, in Skopje. R. Macedonia, Wiebke Werner and Nathanael Raschzok gave oral presentations.
Nathanael Raschzok presented data on  "In vitro evaluation of magnetic resonance imaging contrast agents for labeling of human liver cells" (N. Raschzok, D. A. Muecke, M. Adonopoulou, N. Billecke, A. Zielinski, W. Werner, U. Teichgraeber, I. M. Sauer). Wiebke Werner reported on "Temporal microRNA gene expression profiles of the regenerating rat liver after partial hepatectomy" (W. Werner, N. Raschzok, H. Sallmon, N. Billecke, C. Dame, P. Neuhaus, I. M. Sauer), a joint project of the Department of Neonatology, Charité, and our group.

The 60th Lindau Nobel Laureate Meeting

6a00d83452732869e20133f2863369970b-800wi
Five major scientific institutions in Berlin are celebrating their jubilee in 2010. It is 350 years since the Berlin State Library (Staatsbibliothek zu Berlin) was founded. Three hundred years ago, in 1710, the Charité hospital was erected on the orders of Frederick I, and in the same year the Kurfürstlich Brandenburgische Sozietät der Wissenschaften (Princely Brandenburg Society of the Sciences), which later became the Berlin-Brandenburgische Akademie der Wissenschaften (Berlin Brandenburg Academy of the Sciences), received its first statute. 1810 saw the birth of the Humboldt University, and a century after that it was the turn of the Kaiser Wilhelm Society, the forerunner of the Max Planck Society.
The central exhibition ‘Weltwissen’ (‘Universal Knowledge’), in which all five institutions are taking part, will open at the Martin-Gropius-Bau on 24 September. This gives a panoramic retrospect of 300 years of science in Berlin, from Gottfried Wilhelm Leibniz to the Brothers Humboldt and the Brothers Grimm and continuing on to Albert Einstein and Konrad Zuse. The exhibition “World Knowledge” forms the highlight of the Year of Science. Via illustrative stories of objects, researchers and institutions, the exhibition offers insight into the sciences. It illustrates how scientists have developed Berlin into a metropolis of science with a multitude of institutions and museums. The exhibition is a joint production of the Berlin Brandenburg Academy of Sciences, the Charité Berlin, the Humboldt University of Berlin and the Max-Planck- Society. Partners are: the State Library of Prussian Cultural Heritage Foundation, Free University Berlin, Technical University Berlin, the Museum of Natural History Berlin, die State Museums of Berlin and the German Museum in Munich.
More information via
Berlin - Hauptstadt für die Wissenschaft.

XXIV International Congress of TTS 2012 in Berlin

6a00d83452732869e20133f278dbee970b-800wi
The 2010 Lindau Meeting took place from June 27 till July 2. The 3rd interdisciplinary meeting brought together more than 650 young researchers from around the globe with 59 Nobel Laureates from the fields of physiology or medicine, physics and chemistry.
The  Lindau Nobel Laureate Meetings provide a globally recognised forum for the transfer of knowledge between generations of scientists. They inspire and motivate Nobel Laureates and international Best Talents. Lectures of Nobel Laureates reflect current scientific topics and present relevant fields of research of the future. In panel discussions, seminars and during the various events of the social program young researchers nominated by a worldwide network of Academic Partners interact with Nobel Laureates.
Taking into account the national selection procedures, in excess of 20,000 young researchers apply to attend each Meeting. After Ruth Schwartländer attended in 2008 this year Nathanael Raschzok was chosen and received an invitation to Lindau.

XXXVII ESAO Congress in Skopje

6a00d83452732869e20133f285f711970b-800wi
At the Charité, our scientists and physicians engage in state-of-the-art research, patient care and education. More than half of the German Nobel Prize winners in medicine and physiology come from the Charité, among them Emil von Behring, Robert Koch and Paul Ehrlich. The Charité also has an international reputation for excellence in training. It extends over four campuses with more than 100 clinics and institutes bundled under 17 CharitéCenters. The Charité has a turnover of nearly 1 billion euros per year, and it is one of the largest employers in Berlin with 14,500 employees.
In 2010, the Charité will celebrate its 300-year anniversary and will do everything to make this historical event a memorable one.
Short film covering the Charité - Universitätsmedizin celebrating its 300-year anniversary  (47.91 MB)

XXIII International Congress of TTS

6a00d83452732869e20112791c50a128a4-800wi
Lars Stelter's studies on In vitro and in vivo detectability of modified superparamagnetic nanoparticles for multimodal imaging using fluorescence microscopy, 3T MRI and animal PET are published in the latest issue of Molecular Imaging & Biology (Mol Imaging Biol. 2010 Jan-Feb;12(1):25-34). Co-authors are Jens Pinkernelle, Roger Michel, Ruth Schwartländer, Nathanael Raschzok, Mehmet H. Morgul, Martin Koch, Timm Denecke, Holger Amthauer, Juri Ruf, Andreas Jordan, Bernd Hamm, Igor M. Sauer, Ulf Teichgräber.
Cell transplantation is a major field in regenerative medicine and a promising alternative to whole organ transplantation. However, the process of cell engraftment is not yet fully understood and the hitherto achieved clinical outcome is limited. The aim of our study was to modify an aminosilan-coated nanoparticle for cell labeling and make it applicable for multimodal imaging using MRI, PET and fluorescent imaging. HIV-1 tat, linked FITC, and Gallium-68 were covalently bound to the particle and injected into Wistar rats. Animal-PET imaging was performed followed by MRI at 3.0T. Hepatic accumulation of the particles was proven by radionuclide distribution after 10 minutes in PET as well as in MRI over a 24 hour-period. Histological workup of the liver also revealed content of iron oxide particles in the reticuloendothelial system. Adjacent in vitro studies incubating hepatogenic HuH7 cells with the particles showed a rapid intracellular accumulation, clearly detectable by fluorescence microscopy and MRI. In conclusion our modified nanoparticle is stable under in vitro and in vivo conditions and is applicable for multimodal molecular imaging. Cellular labeling with this particle is possible and might help to get new insights into understanding the process of cell transplantation.

Presentations at GASL 2010

6a00d83452732869e20120a646c438970b-500pi
The XXIII International Congress of The Transplantation Society, the leading international congress on transplantation biology and medicine, will be held in Vancouver, Canada from 15 - 19 August 2010. The translational program will draw together experts from around the world, and will encompass innovations in genomics and proteomics, molecular analyses of human diseases, innovations in biological and pharmacological immunosuppression, novel approaches to tolerance induction, technical advances in robotic surgery and imaging, advances in clinical practice and many other topics at the cutting edge of stem-cell, cellular and solid organ transplantation.
The XXIII Congress will be one of the largest and most exciting international gatherings of clinicians and scientists in the field of transplantation, drawing more than 4,000 delegates from all parts of the world. The international delegation will enjoy a stellar Scientific Program, commencing with a comprehensive and cutting-edge Postgraduate Education Program on Saturday 14th August and Sunday 15th August, and continuing with a panoply of outstanding plenary symposia, state of the art lectures, individual scientific presentations, and thematic symposia from Monday 16th August to Thursday 19th August inclusive.
The 2010 Congress will provide an outstanding opportunity for networking with colleagues and leading experts, and for sharing and discussing clinical advances, late-breaking discoveries and therapeutic advances in this field.
More information is available via 
www.transplantation2010.org. Furthermore, you may download the 2nd announcement here
Important dates:
  • Abstract Submission Opens: October 2009
  • Online Registration Opens: November 2009
  • Abstract Submission Deadline: 5 March 2010
  • Author Notification of Acceptance: 7 May 2010
  • Early Registration Deadline: 4 June 2010
  • Late Breaking Abstract Deadline: 11 June 2010
  • Hotel Reservation Deadline: 25 June 2010
  • Regular Registration Deadline: 16 July 2010

World Conference on Regenerative Medicine 2009

6a00d83452732869e20120a646be5e970b-800wi
Tanja Deurholt's paper on "Novel immortalized human fetal liver cell line, cBAL111, has the potential to differentiate into functional hepatocytes" is published in BMC Biotechnology. Co-authors are Niek P. van Til, Aniska A. Chhatta, Lysbeth ten Bloemendaal, Ruth Schwartlander, Catherine Payne, John N. Plevris, Igor M. Sauer, Robert A.F.M. Chamuleau, Ronald P.J. Oude Elferink, Jurgen Seppen, and Ruurdtje Hoekstra.
A clonal cell line that combines both stable hepatic function and proliferation capacity is desirable for in vitro applications that depend on hepatic function, such as pharmacological or toxicological assays and bioartificial liver systems. The article describes the generation and characterization of a clonal human cell line for in vitro hepatocyte applications.Cell clones derived from human fetal liver cells were immortalized by over-expression of telomerase reverse transcriptase. The resulting cell line, cBAL111, displayed hepatic functionality similar to the parental cells prior to immortalization, and did not grow in soft agar. Cell line cBAL111 expressed markers of immature hepatocytes, like glutathione S transferase and cytokeratin 19, as well as progenitor cell marker CD146 and was negative for lidocaine elimination. On the other hand, the cBAL111 cells produced urea, albumin and cytokeratin 18 and eliminated galactose. In contrast to hepatic cell lines NKNT-3 and HepG2, all hepatic functions were expressed in cBAL111, although there was considerable variation in their levels compared with primary mature hepatocytes. When transplanted in the spleen of immunodeficient mice, cBAL111 engrafted into the liver and partly differentiated into hepatocytes showing expression of human albumin and carbamoylphosphate synthetase without signs of cell fusion. This novel liver cell line has the potential to differentiate into mature hepatocytes to be used for in vitro hepatocyte applications.

ESAO 2009 - presentations

In her Berlin studio, Sarah Illenberger created wonderful delicate knit wool organs. Unfortunately al liver is still pending:

6a00d83452732869e20120a5b04de5970b-pi

Wool organs by visual artist Sarah Illenberger. Photographed by Andreas Achmann for Süddeutsche Zeitung Magazin. 

One Day on the Liver 2009

At the XXXVI Annual Meeting of the European Society for Artificial Organs ESAO 2009  in Compiègne/France the following projects have been presented by members of our group:

  • Tracking of transplanted liver cells via clinical 3.0 tesla MRI A. Zielinski, K. Steinz, N. Raschzok, N. Billecke, N. Kammer, M.H. Morgul, M. Adonopulou, S. Schmeisser, J. Pinkernelle, W. Ruedinger, U. Teichgraeber, I.M. Sauer
  • Evaluation of application sites for liver cell transplantation in a large animal model K. Steinz, A. Zielinski, N. Raschzok, N. Billecke, N.N. Kammer, M.H. Morgul, M. Adonopulou, L. Morawietz, W. Rüdinger, I.M. Sauer
  • Methylprednisolone and tacrolimus prevent hypothermia induced endothelial dysfunction - possible use in transplantation surgery K.R.L. Schmitt, A. Diestel, N. Billecke, F. Berger, I.M. Sauer

HAI 2009 - New aspects in liver support

6a00d83452732869e20120a5b13114970b-800wi
The group was awarded with a research bonus (Forschungsprämie) by the Bundesministeriums für Bildung und Forschung (BMBF, Federal Ministry of Education and Research) supporting the development of micron sized particles for the detection of transplanted cells via MRI. The program is part of the High-Tech Strategy for Germany, launched by the Federal Government in August 2006 to encourage the development of new products and innovative services.

ESAO 2009 - Program online

The Editor-in-Chief Paul S. Malchesky of Artificial Organs informed us that the articles "`Blogs` and `Wikis Are Valuable Software Tools for Communication Within Research Groups" and "Isolation of primary human hepatocytes after partial hepatectomy: criteria for identification of the most promising liver specimen" were in the top 25 articles viewed online in 2008 at Blackwell Synergy.

18. Jahrestagung der DTG in Berlin 2009

6a00d83452732869e20120a607118c970c-320wi
The XXXVI Annual Meeting of the European Society for Artificial Organs ESAO 2009 will take place at the Université de Technologie de Compiègne in Compiègne/France, September 3-5, 2009. 
The program of the ESAO 2009 meeting is now available here .

Molekulare Bildgebung 2009 (MoBi)

6a00d83452732869e20112791e9c2928a4-120pi
As a result of the fruitful collaboration with the Institute for Analytical Sciences Berlin Nathanael Raschzok's paper on "Quantification of Cell Labelling with Micron-Sized Iron Oxide Particles Using Continuum Source Atomic Absorption Spectrometry" has been accepted by Tissue Engineering for publication. Co-authors are Nils Billecke, Nora N. Kammer, Mehmet H. Morgul, Michaela K. Adonopoulou, Igor M. Sauer, Stefan Florek, Helmut Becker-Ross, and Mao-Dong Huang.

Detection of cells after transplantation is necessary for quality control in regenerative medicine. Labelling with micron-sized iron oxide particles (MPIOs) enables non-invasive detection of single cells by magnetic resonance imaging. However, techniques for evaluation of the particle uptake are challenging. The aim of this study was to investigate continuum source atomic absorption spectrometry (CSAAS) for this purpose. Porcine liver cells were labelled with MPIOs and the iron concentration of the cell samples was investigated by a CSAAS spectrometer equipped with a Perkin-Elmer THGA graphite furnace. The weak iron line at 305.754 nm provides only about 1/600 sensitivity of the iron resonance line at 248.327 nm and was used for CSAAS measurements. Iron concentrations measured from labelled cells ranged from (5.8 ± 0.3) to (25.8 ± 0.9) pg Fe/cell, correlating to an uptake of (8.2 ± 0.5) to (25.7 ± 0.8) particles/cell. The results were verified by standardised morphometric evaluation. CSAAS enabled rapid quantification of particle load from small quantities of cells without extensive preparation steps. Thereby, CSAAS could be used for quality control in a clinical setting of cell transplantation.

XXXVI ESAO Congress

6a00d83452732869e20115707cd786970b-800wi
Vom 18. - 20. Juni 2009 findet die Molekulare Bildgebung 2009 (MoBi) in der Charite - Universitätsmedizin Berlin, Campus Virchow-Klinikum statt. Die MoBi ist die jährlich stattfindende Jahrestagung des Interdisziplinären Netzwerkes Molekulare Bildgebung (INMB), das in diesem Jahr von dem Imaging Netzwerk Berlin (INB) in Berlin ausgerichtet wird. Das Programm der Molekularen Bildgebung 2009 verspricht einen interessanten Dialog zwischen jungen und gestandenen Wissenschaftlern aus verschiedenen Disziplinen sowie zwischen Forschung und Industrie. Dabei werden in Schwerpunkt-Sessions unter anderem auch die immer wichtiger werdenden Fragen der Regulation und der klinischen Anwendung molekularer Bildgebungsverfahren thematisiert.
Mehr Informationen via http://www.mobi09.de .

One Day on the Liver 2008 - Programme

6a00d83452732869e2010534d64dbe970b-500pi
The Steering Committee of the Berlin-Brandenburg Center for Regenerative Therapies (BCRT) decided to involve Igor Sauer and his group as an Associated Investigator (AI).
The BCRT is a cooperative research institution of the Charite University Hospital in Berlin and Germany's largest research association, the Helmholtz Association. BCRT also receives generous financial support from the BMBF and the states of Berlin and Brandenburg, as well as from the Technology Foundations in Berlin and Brandenburg, the Future Fund Berlin and from various industry partners. More than 15 regional partners from science and industry are active members of the consortium at the BCRT.

ESAO One Day on the Liver 2008 in Geneva

6a00d83452732869e200e554eb5f4b8833-500pi

ASAIO 2008

aor
Florian Vondran's paper "Isolation of primary human hepatocytes after partial hepatectomy: criteria for identification of the most promising liver specimen." has been published in the March issue of Artificial Organs (Artif Organs 2008, 32: 205-213). Demands for primary human hepatocytes are continuously increasing, while supply is insufficient due to limited cell sources. To improve cell availability, the present study investigates the influence of donor liver characteristics on the outcome of hepatocyte isolation from surgically removed liver tissue (n = 50). Hepatocytes were isolated from liver specimens using a standardized two-step collagenase perfusion technique. The patient's sex, previous chemotherapy, or histopathology have shown no influence. Donor age significantly affected the isolation outcome, but was not found suitable for predicting cell yields. Preoperative blood parameters did not correlate with cell yield, although cell function was affected: total protein, albumin synthesis, and cell viability were significantly decreased for serum gamma-glutamyl-transferase (GGT) levels >60 U/L. Specimens from patients with benign diseases gave significantly higher cell yields than tissue removed due to secondary and primary tumors, respectively. The indication for surgery is a valuable basis for identifying the most yielding specimens. Hepatocytes from donors with high GGT levels appear to show reduced functional properties.

ESAO 2007 - Presentations

logo_esao
During the annual meeting of the ESAO 2007 in Krems Dr. Igor M. Sauer has been elected for the Board of Governors of the European Society for Artificial Organs (ESAO).

ESAO 2007 - One Day on the Liver

logo_esao
Nathanael Raschzok, Haluk Morgül, and Florian Vondran presented their latest results at the XXXIV Congress of ESAO 2007 in Krems, Austria:
  • N. Raschzok, M.H. Morgul, F.W.R. Vondran, R. Schwartlander, I.M. Sauer: The SlideObserver – A new concept for the parallel operation of two SlideReactor bioreactor systems
  • M.H. Morgul, N. Raschzok, R. Schwartlander, F.W.R. Vondran, G. Pless, J. Pinkernelle, U. Teichgraber, I.M. Sauer: Transplantation of primary human hepatocytes – iron oxide labelling for cell detection via MRI
  • F.W.R. Vondran, E. Katenz, R. Schwartlander, M.H. Morgul, N. Raschzok, X. Gong, X. Cheng, P. Neuhaus, I.M. Sauer: Impact of donor liver characteristics on the cell function of primary human hepatocytes

Perspectives in Liver Transplantation

afisbuyuk
The International Medical Students Research Congress (IMSRC) gives students the opportunity to present the results of their scientific research projects. IMSRC 2007 will be held between 11-13 of May, 2007 at Istanbul University, Cerrahpaşa Medical Faculty. Haluk Morgül, Nathanael Raschzok, Keshraw Karmand and Dominik Modest will present their latest results.

International Medical Students Research Congress

groupphoto_small_3

Greetings from Chongqing, PR China

journal-of-biotechnology
Nils Billeckes paper „An operational concept for long-term cinemicrography of cells in mono- and co-culture under highly controlled conditions - the SlideObserver“ was accepted for publication in Journal of Biotechnology! Co-authors are Nathanael Raschzok, Susanne Rohn Mehmet H. Morgul, Ruth Schwartlander, Martina Mogl, Sonja Wollersheim, Katharina R. Schmitt and Igor M. Sauer. The paper is now available online.

Cell morphology, proliferation and motility, as well as mono- and heterotypic cell-to-cell interactions, are of increasing interest for in vitro experiments. However, tightly controlling culture conditions while simultaneously monitoring the same set of cells is complicated. Moreover, video-microscopy of distinct cells or areas of cells over a prolonged period of time represents a technical challenge. The SlideObserver was designed for cinemicrography of cells in co-and monoculture. The core elements of the system are the SlideReactors, miniaturised hollow fibre-based bioreactors operated in closed perfusion loops. Within the SlideReactors, cells can be cultured under standard and experimental conditions as well as in direct- and indirect co-culture. The independent perfusion loops enable controlled variation of parameters such as medium, pH, and oxygenation. A combined automated microscope stage and camera set-up allows for micrograph acquisition of multiple user-defined regions of interest within the bioreactor units. For proof of concept, primary cells (HUVEC, human hepatocytes) and cell lines (HuH7, THP-1) were cultured under stable and varying culture conditions, as well as in mono- and co-culture. The operational system enabled non-stop imaging and automated control of process parameters as well as elective manipulation of either reactor. As opposed to static culture systems or comparable devices for cinemicrographic analysis, the SildeObserver allows simultaneous morphological monitoring of an entire culture of cells in control and experimental bioreactors.

11th WAA & 6th ISFA World Congress

Gesine Pless took another major step in her career by defending her doctoral thesis "magna cum laude". Being (co-)author of more than 16 papers in peer reviewed journals she analysed primary human liver cells from donor organs unsuitable for transplantation cultivated in bioreactors. As each system contains cells originating from an individual organ, each bioreactor culture must be individually characterized. The objective of her study was to identify suitable decisive parameters for the evaluation of cell culture performance.

ESAO 2006 in Umeå, Sweden

cover_1
An ideal extracorporeal liver support system has to provide the main functions of the liver: detoxification, synthesis and regulation. But do we need such an ideal in all clinical situations and can we afford it? The critical issue of the clinical syndrome in liver failure is the accumulation of toxins not cleared by the failing liver. This understanding led to the development of cell-free artificial liver support systems. This special issue of Therapeutic Apheresis and Dialysis is dedicated to cell-free artificial liver support, intended to give engineering scientists, inventors and clinical investigators an update and a better insight into the development and clinical evaluation of cell-free liver support systems. It gives an update on the status quo, both clinically and technically, of different concepts and discusses the current limitations as well as possible solutions. When compared to the cell-based systems, these devices are characterized by easier logistics, lower costs, and absence of hazards related to the cell source. These might be the reasons for being well ahead of the bioartificial concepts in terms of clinical evaluation and application.
Therapeutic Apheresis and Dialysis, April 2006 - Vol. 10 Issue 2:
S. Mitzner et al.: Albumin Regeneration in Liver Support—Comparison of Different Methods J. Patzer: Principles of Bound Solute Dialysis J. Vienken et al.: How Can Liver Toxins be Removed? Filtration and Adsorption With the Prometheus System K. Rifai et al.: Review Article: Clinical Experience With Prometheus J. Rozga et al.: A Novel Plasma Filtration Therapy for Hepatic Failure: Preclinical Studies S.R. Ash: Sorbent Suspensions vs. Sorbent Columns for Extracorporeal Detoxification in Hepatic Failure D. Falkenhagen: Fluidized Bed Adsorbent Systems for Extracorporeal Liver Support L.J. Li: Artificial Liver Support System in China: A Review Over the Last 30 Years R.A.F.M. Chamuleau: Bioartificial Liver: Its Pros and Cons W. Bernal et al.: Cell-Free Artificial Liver Support: Design of Appropriate Clinical Studies with a guest editorial by I.M. Sauer and U. Baurmeister: Cell-Free Artificial Liver Support.

43rd Meeting of the Society for Cryobiology

top_2
The Institut of Biotechnik e.V. together with the Technical University of Munich and Munich University of Applied Sciences cordially invite you to the 5th World Congress of Biomechanics 2006.
Biomechanics covers a wide field from solid to fluid mechanics, from motion sports mechanics to automobile crash tests. It includes tissue engineering and biomaterials, artificial organs and sports therapy. At the 5th World Congress the newest experimental studies will be presented. Special emphasis will be placed on state-of-the art technology and medical applications. Only new, unpublished papers will be accepted.
The 5th World Congress of Biomechanics will explore topics of interest to:
    * physicans
    * bioengineers
    * mechanical and aerospace engineers
    * clinicians in cardiology and other cardiovascular specialties
    * vascular surgeons
    * radiologists
    * neurologists and neurosurgeons
    * orthopedic surgeons
    * specialists in areas such as bone, ligaments, tissue mechanics, biologists,   biochemists, biophysicists, biorheologist

More information via
www.wcb2006.org  .

5th World Congress of Biomechanics 2006

2
After a very successful 1st Basic Course in Laboratory Skills in 2004, you will find attached the Announcement of the 2nd Basic Course in Laboratory Skills, which will take place on October 30 - November 2, 2006, in Rotterdam, The Netherlands.
PhD students, both young physicians and scientists, are encouraged to submit a short letter of motivation and a brief abstract describing their laboratory experiences and PhD project, which has to be sent by e-mail to Dr. Carla C. Baan: .
The application deadline is September 15th, 2006. Goal The course aims to expand the knowledge of laboratory skills within ESOT and to create an environment that stimulates collaboration between scientists to develop tools by which we can monitor rejection and tolerance after organ transplantation.
Hands-on-Course Recent developments of in vitro test systems allow us to study anti-donor responses at large in both experimental models and in patient materials. However, to understand the mechanisms underlying biological phenomena in transplantation, knowledge and practical experience of laboratory techniques is required. The hands on training in laboratory techniques aims to teach young doctors and scientists in molecular biology techniques, flow cytometry based techniques, and functional assays including ELIspot. The educational objective of the course is to learn the laboratory techniques (theory and practice) needed for our understanding of immune responses in experimental transplant models and in patients.

ESOT Basic Science Network

flyer_lswg_esao_2006_1

ESAO 2006 in Umeå, Sweden - Call for abstracts

Dear Colleagues,

I'm pleased to announce that the
World Congress on Medical Physics and Biomedical Engineering 2006 (WC2006) will be held on August 27 to September 1, 2006 in Seoul, Korea. under the Congress Theme: Imaging the Future Medicine. The scientific program will include plenary lectures, sponsored symposia, regular oral and poster sessions.
The Second Announcement, which includes: detailed information on registration procedure, (fees, hotel accommodation, travel information, scientific and social programs), call for papers, as well as registration forms (abstract submission and hotel accommodation) is available via
www.wc2006-seoul.org.
During the congress the scientific track of Artificial Organs will be organized. In order to secure high scientific level of our discipline some of our distinguish colleagues from the ESAO agreed to chair the sessions, that is: Prof. Horst Klinkmann (session: adsorption, apheresis, detoxification), Dr Gerhard Rakhorst (session: cardiac assist devices and cardiovascular implants and prosthesis), and Prof. Dieter Falkenhagen (session: cell implants interactions - biocompatibility). Additionally I have asked to organize some sessions by our colleagues from Japan and USA: Prof. K. Imachi (session: artificial heart), Prof. Yoshida (orthopedic and dental prosthesis and implants) and Prof. D. Klonoff (session: artificial pancreas).
The deadline for abstract submission is 28th of February. I would like to cordially invite you to prepare abstracts as well as to stimulate other researchers to submit appropriate contributions. Think about supporting Artificial Organs scientific track on the biggest congress organized in the field of biomedical engineering and medical physics.

With best regards
Prof. Jan Maria Wojcicki
Cochairman of the track Artificial Organs
For detailed information please contact Ms Mandy Kim - congress manager,
Address: WC 2006 Congress secretariat,
SL. Gangnam P.O.Box 305, Seoul 135-603, Korea, phone: 822-3476- 7700/7723  (Dir), Fax:822-337-6451/3476-8800, E- mail: wc2006@koconex.com

World Congress on Medical Physics and Biomedical Engineering 2006 in Seoul

top_1
The 2006 meeting of the European Society for Artificial Organs (ESAO) will be held in Umea, which is located in the northern part of Sweden, from 21-24 June. At that time, the country celebrates the longest day, a day with 24 hours daylight.
The program is adapted to this biorhytm phenomenon and will start early in the morning (07.30) and will end each day at 16.00 hours. Professor Stegmayr organized various social and sports events that will start after the scientific sessions, to stimulate young researchers to contact with each other and with the experts in the field. Due to the fact that the 2006 congress takes place rather early in the congress season, the deadline for submission of abstracts will be in February 2006. Detailed information will be provided to you in the Second Announcement, that will be mailed to you within a few weeks. For more information please visit the official webpage.
Umea is a very nice city and can be reached be plane from Stockholm easily. The city is the fasted growing town of Sweden. It has approximately 100,000 inhibitants and 30,000 students. Because many members will combine this congress with a short vacation, Professor Stegmayr has organized an unique post congress trip to the Northcape.

2006 ESAO meeting in Umea, Sweden

esot
The Basic Science Committee of ESOT was founded in 2004 with strong support of the ESOT council. The specific aim of the BSC is to support basic science in transplantation within ESOT by enhancing communication, and education and by rasing the profile and quality of basic science at ESOT meetings. One of the key strategies of the BSC is to establish a Europe-wide Basic Science Network in Transplantation where inclusion is dependent on the laboratory/group having five basic science publications in peer-reviewed journals during the previous four years. Details of these laboratories together with selected publications, areas of research interests and specific research tecniques and tools available are available as a link on the ESOT web-site.
In support of one of the BSC strategies, ESOT has made available a number of small Travel Grants (€500 per applicant) to support the travel of individuals wishing to visit a European laboratory outside their own country that can offer a specific technique or expertise. Applications for funding should be made to the members of the ESOT Basic Sciences Committee via the ESOT Secretariat.

ESOT Basic Science Committee Travel Awards

hepatology
The article by Boerries Brandenburg, Lars Stockl, Cindy Gutzeit, Martin Roos, Joachim Lupberger, Ruth Schwartlander, Hans Gelderblom, Igor M. Sauer, Peter Hans Hofschneider, Eberhard Hildt in the December issue of HEPATOLOGY reports on a Novel system for efficient gene transfer into primary human hepatocytes via cell permeable HBV virus-like particle. Protein transduction domains (PTD)s have been used to deliver a variety of biologically active cargo across cellular membranes. However the potential of PTDs to mediate transport of nanoparticular structures into the cytoplasm bypassing the endosomal compartment remains unclear. Based on HBV nucleocapsids cell permeable virus like particles (VLP)s harboring a marker gene were established. Cell permeability was achieved by fusion with TLM-PTD. Electron and confocal microscopy revealed that these VLPs translocate as complete particles across the plasma membrane and transverse the cytoplasm towards the nucleus. Inhibition of endocytosis did not affect translocation of these VLPs into the cytoplasm. Based on these particles a gene transfer system was developed. To this end the particles were loaded with DNA encoding SHBs or eGFP that served as marker genes. Using this system for gene transfer in primary human hepatocytes a gene transfer efficiency of ~95% was observed. In conclusion, the TLM-PTD has the potential to mediate efficient transfer of assembled particles and its cargo i.e. nucleic acids into primary human hepatocytes. This provides the basis for development of novel transducible therapeutic or diagnostic particles. Hepatology 2005, 42 (6): 1300-1309

2nd Swiss Experimental Surgery Symposium

esao_bologna_2
The XXXII Congress of the European Society for Artificial Organs (ESAO) will be held in Bologna from 5 to 8 October, 2005. The Congress will be combined with the first Congress of the International Federation for Artificial Organs (IFAO), which is a newly formed ISAO-derived umbrella structure, embracing ESAO, the American Society for Artificial Internal Organs (ASAIO), the Japanese Society for Artificial Organs (JSAO) and the International Faculty for Artificial Organs (INFA). The XXXII Congress of the European Society for Artificial Organs and the I Congress of the International Federation for Artificial Organs, and the associated Trade Exhibition, will be held at the Palazzo dei Congressi in Bologna, Piazza della Costituzione 4, at the Fiera District, about 15 minutes from the city-center. The final program is available via the ESAO 2005 webpage.
Looking forward to meeting you in Bologna !
The following presentations will give an update on some of our projects:

Oral Presentations The slidereactor – evaluation of a hollow fiber based bioreactor suitable for light microscopy R. Schwartlander, J. Schmid, E. Katenz, X. Cheng, G. Pless, X. Gong, F. Vondran, P. Neuhaus, I.M. Sauer (Berlin, Germany)
Cryopreservation of primary human hepatocytes – protective effect of trehalose? F. Vondran, E. Katenz, X.B. Gong, X.D. Cheng, R. Schwartlander, P. Neuhaus, I.M. Sauer (Berlin, Germany)
First in vitro comparison of two bioartificial liver support systems: MELS CellModule and AMC-BAL P.P. Poyck, G. Pless, R. Hoekstra, S. Roth, A.C.W.A. van Wijk, R. Schwartlander, T.M. van Gulik, I.M. Sauer, R.A. Chamuleau (Amsterdam, The Netherlands; Berlin, Germany)
First results in continuous albumin veno-venous haemodiafiltration in acute liver failure (ALF) H. Ringe, M. Zimmering, W. Luck, I.M. Sauer (Berlin, Germany)
Poster Presentations Acute liver failure: fractionated plasma separation/adsorption (FPSA) and high-flux hemodialysis (HD) - Bridging therapy to LTX G. Junge, S. Kohler, L. Schewior, I.M. Sauer, A. Pascher, P. Neuhaus (Berlin, Germany)
Time-course maintenance of specific functional activity in cultured human hepatocyte E.A. Katenz, F.W.R. Vondran, X.B. Gong, X.D. Cheng, R. Schwartlander, P. Neuhaus, I.M. Sauer (Berlin, Germany)
Effect of matrine on the expression of cytochrome P-450, 2A6, and 2B6 of primary human hepatocytes X. Gong, E.A. Effimova, F.W.R. Vondran, X. Cheng, R. Schwartlander, D. Yang, P. Neuhaus, I.M. Sauer (Berlin, Germany; Guangzhou, Hangzhou, China)

Presentations at the ESAO 2005

Thema: "Tödliche Verwechselungen (Therapie der Konollenblätterpilvergiftung)"
Referenten:
Dr. J. Schefold, Med. Klinik m.S. Nephrologie u. Int. Intensivmedizin, CVK
PD Dr. T. Berg, Med. Klinik m.S. Hepatologie/Gastroenterologie, CVK
Dr. I.M. Sauer, Chirurgie, CVK
Diskutant: Prof. Dr. Dörken, Med. Klinik m.S. Hämatologie/Onkologie

Dienstag, 23.08.2005, 13.00 Uhr im Kursraum 6, Mittelallee 10, Untergeschoss, der Charité - Campus Virchow. Die Veranstaltung ist zertifiziert und ergibt 1 Weiterbildungpunkt der ÄK.

EPGS: One Day On Acute Liver Failure

logo_1
Webcasts of selcted presentations (including videos and slides) of 8th meeting of the Catalan Transplant Foundation in Barcelona 2005 are available via the offical webpage. Some talks deal with the "Tratamiento de la insuficiencia hepática aguda".

GRAND ROUNDS DER MEDIZINISCHEN KLINIKEN

epgs_2
On occasion of the final meeting of the EU-project "Development and clinical study of a bioartificial liver based on reversibly immortalised human hepatocyte" the European Postgraduate Gastro-Surgical School (EPGS) organized the symposium One Day On Acute Liver Failure, to be held at the Academic Medical Center in Amsterdam, on October 28, 2005! Speakers will be K. Boudjema, F. Calise, R.A.F.M. Chamuleau, B. Clement, T.M. van Gulik, R. Hoekstra, R. Jalan, P.L.M. Jansen, E. de Jonge, B. Kreymann, A. Lee, J. Plevris, I.M. Sauer, E. Sokal, and J. Ijzermans. More details and the final program are available here.

Webcast of 8th meeting of CTF

S. Malchesky, the Editor-in-Chief of Artificial Organs, informed us that our article 'Blogs' and 'wikis' are valuable software tools for communication within research groups. was among the top 25 most viewed online in 2005 to date at Blackwell Synergy in general !

ESAO Meeting 2005 in Bologna

kongress_souzhou_2

Greetings from Suzhou, China

bmbf_logo
The German Federal Ministry of Education and Research (BMBF – Bundesministerium für Bildung und Forschung) has awarded a grant of nearly 900.000 € for a new collaborative project in tissue engineering: „Methods for hypothermic long term culture of primary human liver cells in perfusion bioreactors“. Partners are Biochrom AG (Dr. R.K. Schindler), Universitätsklinikum Essen, Institut für Physiologische Chemie (Dr. U. Rauen), and Charité – Universitätsmedizin Berlin, Klinik für Allgemein-, Visceral- und Transplantationschirurgie (Dr. I.M. Sauer).

3rd Int. Symp. on Hepatic failure and Artificial Liver

ao_meeting_1
The Institute of Biomedical engineering, Materials Science and Application (BMSA), and the Department of Surgery (GUMC) organise the symposium: Artificial Organs and Organ Transplantation. 18 February, 2005 - Rode Zaal, Groningen University Medical Center, Groningen, The Netherlands This symposium focuses on highlighting a number of aspects of artificial organs and organ transplantation in general and bridging to new therapies of end stage organ disease in particular. The meeting is supported by the European Society for Artificial Organs (ESAO) annd the European Society for Organ Transplantation (ESOT). Download the program...

Artificial Organs and Organ Transplantation

cover
In Vitro Evaluation of the Transportability of Viable Primary Human Liver Cells Originating From Discarded Donor Organs in Bioreactors by Igor M. Sauer, Ruth Schwartlander, Olav van der Jagt, Ingo Steffen, Ekaterina Efimova, Gesine Pless, Daniel C. Kehr, Dimitrios Kardassis, Jan H. Fruhauf, Joerg C. Gerlach, and Peter Neuhaus (Artificial Organs Volume 29 Issue 2 Page 144-151, February 2005) reports on experiments concerning the impact of two major potential threats to viable cells during transport in hollow fiber bioreactors: temperature changes and mechanical stress.
The use of primary human liver cells obtained from discarded donor organs is increasingly favored for cell-based extracorporeal liver support systems. However, as cryopreservation of primary human hepatocytes causes a significant loss of metabolic activity, the transport of bioreactors with viable liver cells is required. The aim of this study was to evaluate the impact of two major potential threats to viable cells during transport: temperature changes and mechanical stress. Methods: In each experiment three hollow fiber-based bioreactors were charged with primary human liver cells originating from the same discarded donor organ and were simultaneously kept under culture conditions for 8 days. In total, 18 bioreactors were evaluated. On the fifth day the bioreactors were exposed to hypothermia (4°C, n = 3), to hyperthermia (42°C, n = 3), or served as normothermic controls (37°C, n = 3). In a second test series bioreactors were exposed to vibration (21 Hz for 20 min, thereafter 7 Hz for 160 min, n = 3), or were operated as control cultures (n = 6). The release of hepatocyte-specific enzymes was determined as an indicator for cell damage. Results: Hypothermic stress resulted in a significant release of transaminases and led to disturbances of the histological integrity, all indicating a high degree of cell damage. When compared with the control cultures, hyperthermia and mechanical stress in terms of vibration had no significant effect on the cells. Conclusion: The transport of hollow fiber bioreactors charged with viable primary human liver cells appears to be feasible in transport monitors for perfusion and temperature control.  

FALK Symposium No 145: "Artificial Liver Support"

Flyer
For more information on this year's ESAO meeting in Warsaw including the full program please visit http://hrabia.ibib.waw.pl/esao2004/

ESAO 2004: Working Group Liver Support

Clicks-thumb

More than 200 unique visits were registered during the first month. Thanks for all the comments and critics!

Upcoming meetings...

Ruth Schwartlander successfully applied for a grant by the Berliner Graduiertenförderung (NaFöG).

Warsaw ESAO Congress - Abstract submission

Florian Vondran, currently working on his doctoral thesis (cryopreservation of primary human liver cells) qualified for a grant from the Studienstiftung des Deutschen Volkes. As he already receives a grant of the Charité, financial support has been temporarily waived.

Programm–2017