SlideObserver in Journal of Biotechnology

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The combined meeting of World Apheresis Association (WAA) 11 th Congress and 6 th World Congress of the International Society for Apheresis (ISFA) will take place in Pacifico Yokohama Yokohama, Japan, from March 2―4, 2007. WAA federates many national, continental and international societies for hemapheresis and transfusion science, and ISFA is a representative international society for therapeutic apheresis. They have contributed to the development of these scientific fields for many years.
These two societies serve a regular meeting every two years. This is the first combined congress of the two Societies. It is really an anniversary congress. Many lecture, symposium, workshop and seminars are planned in the congress. Highly scientific papers will be presented at free communications and poster sessions. Several supports will be provided to the participants from developing countries. At the same period, 27 th Annual Meeting of Japanese Society for Apheresis (JSFA) will be held in the adjacent venue. I expect the full communication between foreign guests and Japanese participants.
All scientists, researchers, clinicians and co-medicals of are encouraged to submit abstracts and join this anniversary congress.
More information via
the official webpage.

Dr. rer. medic. Ruth Schwartlaender

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Tissue Engineering published the article "Continuously Microscopically Observed and Process-Controlled Cell Culture Within the SlideReactor: Proof of a New Concept for Cell Characterization"  by R. Schwartlander, J. Schmid J, B. Brandenburg, E. Katenz, F.W. Vondran, G. Pless, X. Cheng X, A. Pascher A, P. Neuhaus P, and I.M. Sauer in the January 2007 issue.
Moreover, the editors have chosen one of the figures showing fluorsecent staining of primary human hepatocytes cultured within the SlideReactor as cover-art!
Certain cell types, especially primary human cells, favor a well-defined culture environment offering continuous supply of nutrients and oxygen and waste product removal. Several bioreactors based on special matrices or hollow fibers have been developed that provide such conditions. However, characterization of matrix re-organization or growth of tissue within these systems is possible only after culture termination. Evaluation of the influence of certain medium additives or culture conditions (e.g., temperature, oxygenation) on cell viability, expansion, and differentiation within these systems remains a challenging task. The SlideReactor, a miniaturized hollow fiber-based bioreactor, was developed to enable the observation of cells during culture. An operation concept offering predefined conditions for various cell types has been designed. For proof of concept, primary human cells (hepatocytes, fibroblasts, keratinocytes) and cell lines (HepG2, HuH7, C3A, WiDr, SkHep1) were cultured and observed. A series of experiments (n = 40) showed the feasibility of the set-up; determination of process parameters and continuous observation is possible. The SlideReactor may serve as a simple and cost-efficient tool for cell characterization and optimization of cell-culture conditions.

SlideReactor: Proof of concept

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The paper Cryopreservation of primary human hepatocytes – the benefit of trehalose as an additional cryoprotective agent by Ekaterina Katenz, Florian W.R. Vondran, Ruth Schwartlander, Gesine Pless, Xiaobing Gong, Xiandong Cheng, Peter Neuhaus and Igor M. Sauer is published in the January 2007 issue of Liver Transplantation. Problems with the limited availability of human hepatocytes for cell transplantation may be overcome by efficient cryopreservation techniques and formation of appropriate cell banking. In the present study we investigated the effect of the disaccharide trehalose on the cryopreservation of human hepatocytes. For analysis, liver cells were frozen in culture medium containing 10% DMSO that was supplemented with varying concentrations of trehalose. During the post-thawing culture period, viability, plating efficiency, total protein, cell proliferation, enzyme leakage, albumin and urea formation as well as phase I and II metabolism were analyzed. In the pilot study, among the concentrations investigated, 0.2 M trehalose showed the best overall outcome. Compared to the use of DMSO alone, we found significant improvement in post-thaw cell viability (62.9 ± 13 vs. 46.9 ± 11 %, p < 0.01) and plating efficiency (41.5 ± 18 vs. 17.6 ± 13 %, p < 0.01) in the trehalose group. The use of trehalose as an additive for cryopreserving human hepatocytes resulted in a significantly increased total protein level in the attached cells, higher secretion of albumin and a lower AST level after thawing. The use of trehalose as cryoprotective agent significantly improves the outcome of human hepatocyte cryopreservation.

The SlideReactor

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The Third International Symposium on Hepatic failure and Artificial Liver will be held from Mar 24-27, 2005 in Suzhou, China. The sponsors include Chinese Medical Association Zhejiang Branch and First Affiliated Hospital, Medical College, Zhejiang University. This symposium is aimed to promote the international communication, further the study on artificial liver, popularize the usage of artificial liver system. The conference is focussing on “Developing Artificial Liver, Curing Hepatic Diseases”.

Ruth Schwartlander receives grant

AMC2

In vitro comparison test concerning the MELS CellModule and the AMC-BAL are currently performed in cooperation with the Academisch Medisch Centrum (AMC) in Amsterdam...

SlideReactor starlet at exhibition

A multicompartment SlideReactor is shown at the exhibition “WeltWissen – World Knowledge”.
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This year, Berlin celebrates 200 years of the Humboldt University, 300 years of the Charité, 300 years since the first statute and first publication by the Academy of the Sciences and, one year later, 100 years of the Max Planck and Kaiser Wilhelm Society and the 350th birthday of the Berlin State Library. The exhibition “WeltWissen – World Knowledge” is the high point of the Berlin Year of Science. The Humboldt University, the Charité, the Berlin-Brandenburg Academy of the Sciences and Humanities and the Max Planck Society have organised the exhibition as a unique joint project. The Technical University, the Berlin State Museums and the Deutsches Museum, Munich are involved as partners. From 24 September 2010 to 9 January 2011, Martin-Gropius-Bau will be host   ing its “WeltWissen“ (World Knowledge) exhibition which takes a look at 300 years of the science in Berlin from an all-embracing perspective that crosses institutions, disciplines and epochs. The exhibition is the high point of the Berlin Year of Science. On an exhibition space of more than 3,200 square metres, visitors are presented with over 1,500 original exhibits, installations and media stations. The Humboldt University, the Charité, the Berlin-Brandenburg Academy of the Sciences and Humanities and the Max Planck Society have organised the exhibition as a unique joint project.
The exhibition correlates sciences in Berlin to the world: only the dynamic interplay of local imprinting and worldwide networking has allowed Berlin since 300 years to generate knowledge and share it with the world. Concrete and highly vivid stories and biographies of objects, researchers and institutions offer exciting insights into the scientific environment. “WeltWissen – World Knowledge” shows how scientists in Berlin work, how they network internationally, how they break down the boundaries of their departments and how they transformed Berlin into a scientific metropolis. 

WeltWissen. 300 Years of Science in Berlin 24 September 2010 – 9 January 2011  Martin-Gropius-Bau, Niederkirchnerstrasse 7, 10963 Berlin
Opening times: Wed - Mo: 10.00 am – 8.00 pm, closed on Tuesdays
Admission: 6 €, reduced 4€ . Free admission for children and adolescents up to an including 16 years of age, two escorts each per kindergarten group or school class as well as recipients of unemployment benefit level II 
Public transport: Underground line 2 (Potsdamer Platz), city train lines 1, 2, 25 (Potsdamer Platz or Anhalter Bahnhof), Buses: M29 (Anhalter Bahnhof) / M41 (Abgeordnetenhaus)
Please find more information at: www.weltwissen-berlin.de, www.gropiusbau.de

Copyright of upper,  large picture:
Roman März